Shell vial culture as a tool for isolation of Brucella melitensis in chronic hepatic abscess.

نویسندگان

  • Clarisse Rovery
  • Jean Marc Rolain
  • Didier Raoult
  • Philippe Brouqui
چکیده

A 50-year-old man was admitted with a liver abscess and positive serology for Brucella spp. Liver pus and blood cultures remained sterile on conventional culture. Inoculation of liver pus onto eukaryotic cells by the centrifugation-shell vial technique yielded Brucella melitensis, identified by 16S rRNA gene amplification and sequencing.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Isolation, cloning and expression of the Brucella melitensis Omp31 gene

Brucellosis is a zoonotic disease transmitted to humans either from animals or from their products.Although brucellosis can be found worldwide, the Mediterranean Basin, South and Central America, EasternEurope, Asia, Africa, the Caribbean, and the Middle East have currently been listed as high-risk regions. Thegenus Brucella is classified in at least nine species. Brucella melitensis is the glo...

متن کامل

A Rare Case Report On Skin Abscess In Brucellosis In Children

Brucellosis is an acute and chronic disease of cattle and can be transmitted to human beings. This disease is associated with numerous clinical manifestations and the rate of incidence of its skin complications is 5% in the affected patients. One of its rare skin complications is brucella abscess. In this study a 9-years old girl is presented with skin abscess of the posterior part of right ank...

متن کامل

Amplification, cloning and expression of Brucella melitensis bp26 gene (OMP28) isolated from Markazi province (Iran) and purification of Bp26 Protein

Brucellosis is a debilitative disease that imposes costs on both economy and society. It is shown that although the vaccine can prevent abortion, it does not provide complete protection against infection. In Iran, Brucella melitensis is a common causative agent for brucellosis and BP26 protein of this bacterium having a good antigenesity and an important vaccine candidate. In this study B. meli...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of clinical microbiology

دوره 41 9  شماره 

صفحات  -

تاریخ انتشار 2003